Spatial and temporal expression patterns of Atoh1 during mice cerebellar development / 解剖学报
Acta Anatomica Sinica
; (6): 134-141, 2023.
Article
in Zh
| WPRIM
| ID: wpr-1015229
Responsible library:
WPRO
ABSTRACT
Objective To investigated the spatial and temporal expression of Atoh1 mRNA and protein level during the cerebellar development. Methods The frozen cerebellum sections of mice at different developmental stages were used to analyze the temporal and spatial expression patterns of Atoh1 mRNA by RNA scope technique, n= 3 for each group. Meanwhile, two reporter mice with different genetic modification method were utilized to analyze the spatial and temporal expression of Atoh1 at the protein level by immunofluorescence staining, n= 3 for each group. Results Atoh1 mRNA was highly expressed in the embryonic cerebellar rhombic lip (RL) and the external granule layer (EGL). At the protein level, staining result of Atoh1-3
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WPRIM
Language:
Zh
Journal:
Acta Anatomica Sinica
Year:
2023
Type:
Article