Effect of alkaline phosphatase liver / bone / kidney overexpression on ventricular remodeling after acute myocardial infarction in mice / 解剖学报

ABSTRACT

Objective To investigate the effect of alkaline phosphatase liver / bone / kidney (ALPL) overexpression on ventricular remodeling after acute myocardial infarction (MI). Methods Thirty six 8-week-old male C57BL / 6 J mice were randomly divided into sham group(sham), MI group (MI+Adv-EGFP) and ALPL overexpression group (MI+ Adv-ALPL) with 12 mice in each group. Two weeks after MI, cardiac function of mice was detected by echocardiography, pathological changes was detected by HE staining. ALPL mRNA expression in mouse heart was detected by Real-time PCR. The levels of ALPL, α- smooth muscle actin (α-SMA),collagen I (ColI) and collagen III (ColIII) protein were detected by Western blotting. The collagen volume fraction (CVF) and the ratio of Col I / Col III were measured by polarizing method with Sirius red staining. Results Compared with MI+Adv-EGFP group, the left ventricular ejection fraction (LVEF) (%) and left ventricular fractional shortening (LVFS) (%) of the heart in MI+Adv-ALPL group were reduced significantly (P < 0. 05). Hearts in the ALPL overexpression group showed myocardial fiber broken, and disordered and fibrous scars were obvious in myocardial infarction area. The expression of ALPL and the expression of α-SMA, ColI and ColIII protein in MI+Adv-ALPL group increased significantly (P<0. 05), and the proportion of ColI / ColIII in MI+Adv-ALPL group increased significantly (P<0. 05).Compared with MI+Adv-EGFP group, the CVF and the proportion of the ratio of ColI / ColIII in the MI+Adv-ALPL group increased significantly (P<0. 05). Conclusion ALPL overexpression can promote the ventricular pathological remodeling after acute myocardial infarction in mice.