The Interaction between Aptamer and Beclomethasone Based on High Resolution Mass Spectrometry and Molecular Docking / 中国生物化学与分子生物学报
Chinese Journal of Biochemistry and Molecular Biology
;
(12): 636-643, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-1015944
ABSTRACT
Beclomethasone is an effective glucocorticoid, and beclomethasone-aptamer is a short single-stranded DNA with affinity and specificity to beclomethasone. The interaction between them is still unclear. The study of the interaction between aptamers and beclomethasone has a certain significance for the application of aptamers. In this study, high-resolution Fourier transform ion cyclotron resonance mass spectrometer (FT-MS) and molecular docking simulation technology were used to study the interaction between aptamer and beclomethasone. Firstly, under the optimized conditions of high-resolution mass spectrometry parameters, the complex of aptamer and beclomethasone was detected by the negative ion scanning mode with the electrospray ion source. Based on the results of high-resolution mass spectrometry, most of the compounds were -8-valent ions, and their dissociation constant K
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Biochemistry and Molecular Biology
Year:
2021
Type:
Article
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