Expression of Circ-RANBP1 in Pancreatic Cancer Tissue and its Effect on Cell Proliferation, Migration and Invasion / 胃肠病学

ABSTRACT

Background: Studies have shown that abnormal expression of circular RNA (circRNA) is closely related to the development, progress and prognosis of a variety of tumors, and is an ideal diagnostic indicator and therapeutic target. However, the role of circRNA in the development and progress of pancreatic cancer needs to be further explored. Aims: To investigate the expression of circ-RANBP1 in pancreatic cancer tissue and its effect on pancreatic cancer cell proliferation, migration and invasion. Methods: The expression of circ-RANBP1 in pancreatic cancer tissue and normal para-cancerous tissue was detected by in situ hybridization. MIA PaCa-2 cells and SW 1990 cells were cultured, and transfected with knockdown oligomer and overexpressed plasmid of circ-RANBP1, respectively, and corresponding control groups were served. Expression of circ-RANBP1 in pancreatic cancer cells was detected by qRT-PCR. EdU method was used to detect the effect of circ-RANBP1 on cell proliferation. Transwell assay was used to detect the effect of circ-RANBP1 on cell migration and invasion. Western blotting and immunofluorescence were used to detect the effect of circ-RANBP1 on epithelial-mesenchymal transition (EMT). Angiogenesis assay was used to explore the effect of circ-RANBP1 on angiogenesis ability. Results: The expression of circ-RANBP1 was significantly increased in pancreatic cancer tissue when compared with paired normal tissues, and was closely associated with poor prognosis of patients. Circ-RANBP1 knockdown inhibited the proliferation of MIA PaCa-2 cells, while overexpression of circ-RANBP1 promoted the proliferation of SW 1990 cells. Compared with control group, circ-RANBP1 knockdown suppressed the migration and invasion of MIA PaCa-2 cells, and overexpression of circ-RANBP1 promoted the migration and invasion of SW 1990 cells. Knockdown of circ-RANBP1 could inhibit EMT, while circ-RANBP1 overexpression showed opposite effect. Inhibition of circ-RANBP1 significantly reduced angiogenesis, while overexpression of circ-RANBP1 significantly enhanced angiogenesis. Conclusions: Circ-RANBP1 is highly expressed in pancreatic cancer tissue, and can promote the proliferation, migration, invasion, EMT and angiogenesis of pancreatic cancer cells.