Hypoxia Delays the Intracellular Ca2+ Clearance by Na+ - Ca2+ Exchanger in Human Adult Cardiac Myocytes
Yonsei Medical Journal
; : 333-337, 2001.
Article
in En
| WPRIM
| ID: wpr-101706
Responsible library:
WPRO
ABSTRACT
Transient myocardial ischemia during cardiac surgery causes a loss of energy sources, contractile depression, and accumulation of metabolites and H+ ion resulting in intracellular acidosis. The reperfusion following ischemic cardioplegia recovers intracellular pH, activates Na+-H+ exchange and Na+-Ca2+ exchange transports and consequently produces Ca2+ overload, which yields cell death. Among the various Ca2+ entry pathways, the Na+-Ca2+ exchanger is known to play one of the major roles during the ischemia/reperfusion of cardioplegia. Consequently, information on the changes in intracellular Ca2+ activities of human cardiac myocytes via the Na+-Ca2+ exchanger is imperative despite previous measurements of Ca2+ current of human single myocytes. In this study, human single myocytes were isolated from the cardiac tissues obtained during open-heart surgery and intracellular Ca2+ activity was measured with cellular imaging techniques employing fluorescent dyes. We report that the Na+-Ca2+ exchanger of adult cardiac myocytes is more susceptible to hypoxic insult than that of young patients.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Calcium
/
Sodium-Calcium Exchanger
/
Hydrogen-Ion Concentration
/
Hypoxia
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Middle Aged
Limits:
Adult
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Child
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Child, preschool
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Female
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Humans
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Infant
/
Male
Language:
En
Journal:
Yonsei Medical Journal
Year:
2001
Type:
Article