Effect of the Flavonoid Luteolin for Dextran Sodium Sulfate-induced Colitis in NF-kappa BEGFP Transgenic Mice / 영남의대학술지
Yeungnam University Journal of Medicine
;
: 26-35, 2006.
Article
in Korean
| WPRIM
| ID: wpr-102198
ABSTRACT
BACKGROUND:
Luteolin, a flavone found in various Chinese herbal medicines is known to possess anti-inflammatory properties through its ability to inhibit various proinflammatory signaling pathways including NF-kappa B and p38 MAPK. In this study, we investigated the potential therapeutic effect of luteolin on dextran sodium sulfate (DSS)-induced colitis. MATERILAS ANDMETHODS:
We used a transgenic mouse model expressing the enhanced green fluorescent protein (EGFP) under the transcriptional control of NF-kappa B cis-elements. C57BL/6 NF-kappa BEGFP mice received 2.5% DSS in their drinking water for six days in combination with daily luteolin administration (1mg/kg body weight, 0.1ml vol, intragastric) or vehicle. NF-kappa B activity was assessed macroscopically with a Charge-Coupled Device (CCD) camera and microscopically by confocal analysis.RESULTS:
A significant increase in the Disease Activity Index (DAI), histological score (p<0.05), IL-12 p40 secretion in colonic stripe culture (p<0.05) and EGFP expression was observed in luteolin and/or DSS-treated mice compared to water-treated mice. Interestingly, a trend toward a worse colitis (DAI, IL-12p40) was observed in luteolin-treated mice compared to non-treated DSS-exposed mice. In addition, EGFP expression (NF-kappa B activity) strongly increased in the luteolin-treated mice compared to control mice. Confocal microscopy showed that EGFP positive cells were primarily lamina propria immune cells.CONCLUSIONS:
These results suggest that luteolin is not a therapeutic alternative for intestinal inflammatory disorders derived for primary defects in barrier function. Thus, therapeutic intervention targeting these signaling pathways should be viewed with caution.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sodium
/
Drinking Water
/
Body Weight
/
Mice, Transgenic
/
NF-kappa B
/
Dextrans
/
Colitis
/
Colon
/
Microscopy, Confocal
/
Interleukin-12
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Yeungnam University Journal of Medicine
Year:
2006
Type:
Article
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