Effect of antioxidants on cyclooxygenase-2 expression, prostaglandin synthesis and nuclear factor Kappa-B activity in human myometrium / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology
;
: 2103-2111, 2005.
Article
in Korean
| WPRIM
| ID: wpr-102610
ABSTRACT
OBJECTIVE:
The oxidative stimuli activate NF-kappa B, which is a transcriptional factor inducing the expression of cyclooxygenase-2 (COX-2), an enzyme which involves in the rate-limiting step of prostaglandin (PG) synthesis. This study was performed to investigate whether vitamin C or caffeic acid phenethyl ester (CAPE) suppress COX-2 protein expression, PG synthesis, and NF-kappa B activity in human myometrium at term.METHODS:
Human myometrial cells were cultured and stimulated with tumor necrosis factor-alpha (TNF-alpha), and also treated with TNF-alpha plus caffeic acid phenethyl ester, and TNF-alpha plus vitamin C. The expressions of COX-2 protein were assessed by western blot analyses. The activity of NF-kappa B was measured by luciferase assay. PGE2 production was determined by ELISA.RESULTS:
The expression of COX-2 protein and the synthesis of PGE2 were promoted by TNF-alpha and peaked after 6 hours of exposure. CAPE and vitamin C significantly inhibited the TNF-alpha-dependent expression of COX-2 protein and the synthesis of PGE2. NF-kappa B activities were also suppressed by the addition of CAPE and vitamin C to TNF-alpha pre-stimulated cells.CONCLUSION:
The antioxidants, CAPE and vitamin C, inhibited COX-2 expression, PGE2 synthesis and NF-kappa B activity in human myometrial cells. These results suggest that the antioxidant may play an inhibitory role in human labor.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ascorbic Acid
/
Enzyme-Linked Immunosorbent Assay
/
Dinoprostone
/
Blotting, Western
/
NF-kappa B
/
Tumor Necrosis Factor-alpha
/
Cyclooxygenase 2
/
Luciferases
/
Myometrium
/
Antioxidants
Limits:
Animals
/
Female
/
Humans
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2005
Type:
Article
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