Detection of O-Linked-N-Acetylglucosamine Modification and Its Associated Enzymes in Human Degenerated Intervertebral Discs
Asian Spine Journal
;
: 863-869, 2017.
Article
in English
| WPRIM
| ID: wpr-102663
ABSTRACT
STUDY DESIGN:
Human herniated discs were obtained from discectomy specimens for the immunohistochemical detection of O-GlcNAc and O-GlcNAcase (OGA)/O-GlcNAc transferase (OGT).PURPOSE:
This study aimed to quantify the extent of O-GlcNAcylation and its associated enzymes (OGT/OGA) in human degenerated intervertebral discs. OVERVIEW OF LITERATURE The O-GlcNAcylation of nuclear, cytoplasmic, and mitochondrial proteins as well as the effects of such post-translational modifications are currently the focus of extensive research. O-GlcNAcylation is believed to contribute to the etiology of chronic illnesses by acting as a nutrient and stress sensor in the cellular environment. Mature intervertebral disc cells are chondrocyte-like cells, and O-GlcNAc has been shown to promote chondrocyte apoptosis in vitro. We believe that O-GlcNAcylation is a key regulator of disc degeneration.METHODS:
Fifty-six specimens were fixed for 24 hours in a 10% solution of neutral-buffered formaldehyde, dehydrated, and embedded in paraffin. Tissue slices (4-µm-thick) were used for hematoxylin-eosin staining and immunohistochemistry.RESULTS:
We found that O-GlcNAcylation of cytoplasmic proteins was less than that of nuclear proteins in both single cells and cell clusters. Cytoplasmic O-GlcNAcylation occurs subsequent to nuclear O-GlcNAcylation and is directly proportional to disc degeneration. OGT and O-GlcNAc expression levels were identical in all specimens examined.CONCLUSIONS:
O-GlcNAc and OGA/OGT expression is shown to correlate for the first time with intervertebral disc cell degeneration. Increasing disc degeneration is associated with increasing O-GlcNAcylation in both nuclear and cytoplasmic proteins in human disc cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Paraffin
/
Spine
/
Transferases
/
In Vitro Techniques
/
Immunohistochemistry
/
Nuclear Proteins
/
Protein Processing, Post-Translational
/
Chronic Disease
/
Apoptosis
/
Diskectomy
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Asian Spine Journal
Year:
2017
Type:
Article
Similar
MEDLINE
...
LILACS
LIS