Distribution of CD4+CD25+ T cells and graft-versus-host disease in human hematopoietic stem cell transplantation / 소아과
Korean Journal of Pediatrics
;
: 1336-1341, 2008.
Article
in Korean
| WPRIM
| ID: wpr-103105
ABSTRACT
PURPOSE:
This study aimed to determine the frequencies of CD4+CD25+ T cells in donor graft and peripheral blood CD4+CD25+ T cells in recipients after hematopoietic stem cell transplantation (HSCT) and their association with graft-versus-host disease (GVHD).METHODS:
Seventeen children who underwent HSCT were investigated. CD4+CD25+ T cells in samples from donor grafts and recipient peripheral blood were assessed by flow cytometry at 1 and 3 months after transplantation.RESULTS:
CD4+CD25+ T cell frequencies in the grafts showed no significant difference between patients with and without acute GVHD (0.90% vs. 1.06%, P=0.62). Absolute CD4+CD25+ T cell number in grafts were lower in patients with acute GVHD than in those without acute GVHD (6.18x10(5)/kg vs. 25.85x10(5)/kg, P=0.09). Patients without acute GVHD showed a significant decrease in peripheral blood CD4+CD25+ T cell percentage at 3 months compared to those at 1 month after HSCT (2.11% vs. 1.43%, P=0.028). However, in patients with acute GVHD, CD4+CD25+ T cell percentage at 3 months was not different from the corresponding percentage at 1 month after HSCT (2.47% vs. 2.30%, P=0.5).CONCLUSION:
The effect of frequencies of CD4+CD25+ T cells in donor grafts on acute GVHD after HSCT could not be identified, and the majority of peripheral blood CD4+CD25+ T cells in patients who underwent HSCT may be activated T cells related to acute GVHD rather than regulatory T cells. Further studies with additional markers for regulatory T cells are needed to validate our results.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tissue Donors
/
Hematopoietic Stem Cells
/
T-Lymphocytes
/
Cell Count
/
T-Lymphocytes, Regulatory
/
Hematopoietic Stem Cell Transplantation
/
Transplants
/
Flow Cytometry
/
Graft vs Host Disease
Limits:
Child
/
Humans
Language:
Korean
Journal:
Korean Journal of Pediatrics
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS