Evaluation of Genedia HBsAg Rapid and Genedia Anti-HBs Rapid for the Screening of HBsAg and Anti-HBs / 대한임상병리학회지
Korean Journal of Clinical Pathology
;
: 114-117, 1999.
Article
in Korean
| WPRIM
| ID: wpr-103161
ABSTRACT
BACKGROUND:
We evaluated a rapid screening kit for the detection of hepatitis surface antigen (HBsAg) and antibody (anti-HBs) using an immunochromatographic method. MATERIALS ANDMETHODS:
We selected 499 serum specimens for the evaluation. Each specimen was tested by enzyme immunoassay (EIA; Cobas Core, Roche, Switzerland), reverse passive hemagglutination (RPHA; Serodia HBs, Asan, Korea) for HBsAg, passive hemagglutination (PHA; Serodia Anti-HBs, Asan, Korea) for anti-HBs, and with the Genedia HBsAg and Anti-HBs Rapid (Green Cross Corp., Korea) kits. Results of each assay were compared with those of the EIA.RESULTS:
The sensitivities and specificities of the Genedia HBsAg Rapid kit were 98.0% and 100%, and those for the Genedia Anti-HBs Rapid kit were 95.3% and 98.0%, respectively. These were higher than those for RPHA (96.0% and 100%), and PHA (83.2% and 96.0%). Concordance rates between EIA and Genedia HBsAg Rapid, Genedia Anti-HBs Rapid, RPHA, and PHA were 98.8%, 96.4%, 97.6%, and 88.4%, respectively. Extending the incubation time from the recommended 30 minutes to 2 hours increased the sensitivities of the Genedia kits.CONCLUSION:
The Genedia HBsAg and Anti-HBs Rapid kits are simple, sensitive, and inexpensive assays suitable for screening or use in emergency situations.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Mass Screening
/
Immunoenzyme Techniques
/
Emergencies
/
Hemagglutination
/
Hepatitis
/
Hepatitis B Surface Antigens
/
Antigens, Surface
Type of study:
Diagnostic study
/
Screening study
Language:
Korean
Journal:
Korean Journal of Clinical Pathology
Year:
1999
Type:
Article
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