Tracking Intravenous Adipose-Derived Mesenchymal Stem Cells in a Model of Elastase-Induced Emphysema / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
;
: 116-123, 2014.
Article
in English
| WPRIM
| ID: wpr-103201
ABSTRACT
BACKGROUND:
Mesenchymal stem cells (MSCs) obtained from bone marrow or adipose tissue can successfully repair emphysematous animal lungs, which is a characteristic of chronic obstructive pulmonary disease. Here, we describe the cellular distribution of MSCs that were intravenously injected into mice with elastase-induced emphysema. The distributions were also compared to the distributions in control mice without emphysema.METHODS:
We used fluorescence optical imaging with quantum dots (QDs) to track intravenously injected MSCs. In addition, we used a human Alu sequence-based real-time polymerase chain reaction method to assess the lungs, liver, kidney, and spleen in mice with elastase-induced emphysema and control mice at 1, 4, 24, 72, and 168 hours after MSCs injection.RESULTS:
The injected MSCs were detected with QD fluorescence at 1- and 4-hour postinjection, and the human Alu sequence was detected at 1-, 4- and 24-hour postinjection in control mice (lungs only). Injected MSCs remained more in mice with elastase-induced emphysema at 1, 4, and 24 hours after MSCs injection than the control lungs without emphysema.CONCLUSION:
In conclusion, our results show that injected MSCs were observed at 1 and 4 hours post injection and more MSCs remain in lungs with emphysema.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spleen
/
Bone Marrow
/
Adipose Tissue
/
Pulmonary Disease, Chronic Obstructive
/
Quantum Dots
/
Emphysema
/
Cell Tracking
/
Mesenchymal Stem Cells
/
Real-Time Polymerase Chain Reaction
/
Optical Imaging
Limits:
Animals
/
Humans
Language:
English
Journal:
Tuberculosis and Respiratory Diseases
Year:
2014
Type:
Article
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