Effect of miR 27a targeting regulation of SFRP1 on biological behavior of colorectal cancer / 安徽医科大学学报

ABSTRACT

Objective @#To investigate the expression of miR-27a in colorectal cancer cell , and to analyze the effect of its targeted regulation of ( Secreted Frizzled Related Protein , SFRP1) on the biological behavior of colorectal cancer cells .@*Methods @#Real time fluorescent quantitative PCR(qRT-PCR) was employed to examine the expres sion of miR-27a and SFRP1 mRNA in colorectal cancer tissues and adjacent normal tissues . Western blot was used to detect the expression of SFRP1 protein in colorectal cancer tissues and adjacent normal tissues . TargetScan soft ware and dual luciferase reporter gene test were used to detect the targeted regulation of miR-27a on SFRP1 . HCT116 cells were transfected with miR-27a mimic , miR-27a inhibitor and negtive control (NC) . The expression of miR-27a and SFRP1 mRNA in each group was determined by qRT-PCR . MTT colorimetry was performed to eval uate the proliferation of each group cells . Transwell assay was used to evaluate the cell invasion and migration ability . Meanwhile , the protein expression levels of SFRP1 , key factors Wnt4 and β-catenin in the Wnt/β catenin signaling pathway were determined by Western blot.@*Results @#Compared with adjacent normal tissues , miR-27a was highly expressed in colorectal cancer tissues , while SFRP1 was low expressed in colorectal cancer tissues ( P < 0.05) . Target Scan software and dual luciferase reporter gene test showed that miR-27a targeted SFRP1 . Compared with NC group , the expression of miR-27a of miR-27a mimic group increased , the proliferation , invasion and migration ability enhanced , the expression of SFRP1 protein decreased , while Wnt4 and β-catenin protein expression increased ( P < 0.05 ) . Compared with miR-27a mimic group , the expression of miR-27a of miR-27a inhibitor group decreased , the proliferation , invasion and migration ability reduced , the expression of SFRP1 protein in creased , while Wnt4 and β-catenin protein expression decreased( P < 0.05) .@*Conclusion @#miR-27a can target SFRP1 , inhibit the proliferation , invasion and migration of colorectal cancer cells , mainly by up regulating SFRP1 and blocking the downstream Wnt/β-catenin signaling pathway , which provides a new direction for clinical treatment.