Study on development of DNA probe for identification of Prevotella intermedia G8-9K-3 / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
;
: 281-290, 2002.
Article
in Korean
| WPRIM
| ID: wpr-103313
ABSTRACT
The purpose of this study is to develop species-specific DNA probe for detection and identification of Prevotella intermedia (P. intermedia) G8-9K-3. This study procedure includes (1) whole-genomic DNA extraction of P. intermedia G8-9K-3 (2) construction of the genomic DNA library, (3) screening of strain-specific DNA probe by reverse dot hybridization, (4) confirmation of strain-specific DNA probe by Southern blot hybridization, (5) determination of nucleotide sequences of strain-specific DNA probe. Twenty-eight recombinant plasmids containing Hind III-digested DNA fragments of P. intermedia G8-9K-3 were obtained. Reverse dot Hybridization and Southern blot analysis data showed that one of them, Pig3, could be P. intermedia G8-9K-3-specific DNA probe. This datum indicates that this Pig3 DNA probe could be useful in detection and identification of the P. intermedia G8-9K-3 strain.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
DNA
/
Base Sequence
/
Gene Library
/
Mass Screening
/
Blotting, Southern
/
Prevotella intermedia
/
Prevotella
Type of study:
Diagnostic study
/
Screening study
Language:
Korean
Journal:
The Journal of the Korean Academy of Periodontology
Year:
2002
Type:
Article
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