Relationship between Poor Immunogenicity of HLA-A2-Restricted Peptide Epitopes and Paucity of Naive CD8+ T-Cell Precursors in HLA-A2-Transgenic Mice
Immune Network
;
: 219-225, 2014.
Article
in English
| WPRIM
| ID: wpr-103514
ABSTRACT
We examined the immunogenicity of H-2 class I-restricted and HLA-A2-restricted epitopes through peptide immunization of HLA-A2-transgenic mice that also express mouse H-2 class I molecules. All four of the tested epitopes restricted by H-2 class I robustly elicited T-cell responses, but four of seven epitopes restricted by HLA-A2 did not induce T-cell responses, showing that HLA-A2-restricted peptide epitopes tend to be poorly immunogenic in HLA-A2-transgenic mice. This finding was confirmed in HLA-A2-transgenic mice infected with a recombinant vaccinia virus expressing hepatitis C virus proteins. We examined the precursor frequency of epitope-specific naive CD8+ T cells in HLA-A2-transgenic and conventional C57BL/6 mice and found that the poor immunogenicity of HLA-A2-restricted peptide epitopes is related to the paucity of naive CD8+ T-cell precursors in HLA-A2-transgenic mice. These results provide direction for the improvement of mouse models to study epitope repertoires and the immunodominance of human T-cell responses.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Vaccinia virus
/
T-Lymphocytes
/
HLA-A2 Antigen
/
Immunization
/
Hepacivirus
/
Epitopes, T-Lymphocyte
/
Precursor Cells, T-Lymphoid
/
Epitopes
Limits:
Animals
/
Humans
Language:
English
Journal:
Immune Network
Year:
2014
Type:
Article
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