Effects of aflatoxin B1 on the biophysical properties and cytoskeleton structure of hepatocellular carcinoma cell line HepG2 / 安徽医科大学学报
Acta Universitatis Medicinalis Anhui
; (6): 10-14, 2023.
Article
in Zh
| WPRIM
| ID: wpr-1036370
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WPRO
ABSTRACT
Objective @#To investigate the effects of aflatoxin B1 (AFB1) on the biophysical properties and cytoskeleton structure of human hepatocellular carcinoma cells (HCCs) . @*Methods@#HepG2 cells were respectively treated with 0 , 0. 01 , 0. 1 , 1 , 5 , 10 μmol/L AFB1 for 24 h and 48 h , and the cell viability was measured by CCK⁃8 kit.Based on this result , the influences of 10 μmol/L AFB1 on the osmotic fragility , membrane fluidity , electrophoretic mobility (EPM) and F ⁃actin structure of cells were analyzed. Subsequently , total RNAs were extracted and the PCR. @*Results@#The increased viability of HepG2 cells was induced by AFB1 in a dose⁃dependent manner after 48h treatment. After treated with 10 μmol/L AFB1 , the anti⁃hypotonic ability and EPM of HepG2 cells were en⁃hanced. The content of F ⁃actin in HepG2 cells increased obviously , while the mRNA expression levels of the main cytoskeleton binding proteins were altered. @*Conclusion @#AFB1 can affect the biophysical properties , cytoskeleton structure and its binding proteins of HepG2 cells , which may be directly related to its toxic action.
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Language:
Zh
Journal:
Acta Universitatis Medicinalis Anhui
Year:
2023
Type:
Article