Clinical Application of Combined Detection of AnuA,ANA,anti ds-DNA and anti-ENA in SLE Patients / 中国现代医生

ABSTRACT

Objective To combine detection of SLE patients with a variety of autoantibodies, provide a basis for clinical diagnose and treat-ment. Methods AnuA,anti-Sm,anti-SSA,anti-SSB and anti-Rib were determined by western blotting,and anti-ds-DNA and ANA were determined by indirect immunofluorescence(IIF) in 120 cases of SLE patients and 60 controls. Results The positive rates of ANA,anfi ds-DNA, AunA, anti-Sm, anti-SSA, anti-SSB and anti-Rib was 69.2%, 70.8%, 74.2%, 35%, 42.5%, 11.7% and 26.7% in SLE patients. The positive rates of ANA,anti ds-DNA and AunA was 90.7%,98.1% and 83.3% in patients with active stage of SLE. The positive rates of ANA, anti ds-DNA and AunA was 51.5%,48.5% and 66.7% in patients with inactive stage of SLE. Three indicators had statistically signitieance in active stage and inactive stage(P<0.01,P< 0.01 and P< 0.05). All of the test results of 60 controls were negative. Conclusion Combined detection of several autoantibody was important to diagnosis and curative evaluation in SLE.