Effects of Heme Oxygenase-1 Expression in Mycophenolic Acid Induced Apoptosis of Jurkat Cell Lines
Journal of the Korean Surgical Society
;
: 343-349, 2010.
Article
in Korean
| WPRIM
| ID: wpr-10366
ABSTRACT
PURPOSE:
This study demonstrates that pharmacologic induction of heme oygenase-1 (HO-1) along with catalytic activation significantly modulated apoptosis of Jurkat cells induced by mycophenolic acid (MPA).METHODS:
Cells were cultured with the presence or absence of MPA. Flow cytometric analysis was performed after propidium iodide staining. Western blotting of HO-1, Bcl, and Bax was also performed. Cells were stained 4'-6-Diamidino-2-phenylindole (DAPI) and measured by flow cytometry in the absence or presence of CoPPIX.RESULTS:
Treatment of MPA decreased cell viability in a dose- and time-dependent manner. MPA-induced cell death was confirmed as apoptosis characterized by sub G0/G1 phase arrest. Expression of HO-1 assumes a pattern of decline after rising at the initial phase. CoPPIX, HO-1 inducer, significantly inhibited the cisplatin-induced apoptosis. Treatment of MPA resulted in reactive oxygen species (ROS) generation in Jurkat cells. CoPPIX attenuated ROS production in MPA-treated cells.CONCLUSION:
This result suggests that the protective mechanism of HO-1 on MPA-induced cytotoxicity is associated with direct inhibition of ROS generation and mitochondrial permeability transition.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Permeability
/
Propidium
/
Cell Survival
/
Blotting, Western
/
Cell Death
/
Reactive Oxygen Species
/
Apoptosis
/
Jurkat Cells
/
Heme Oxygenase-1
/
Flow Cytometry
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Surgical Society
Year:
2010
Type:
Article
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