A New Gene Regulated by Electroconvulsive Shock in Rat Brains / 신경정신의학
Journal of Korean Neuropsychiatric Association
;
: 928-935, 2000.
Article
in Korean
| WPRIM
| ID: wpr-103924
ABSTRACT
OBJECTIVES:
This study was performed to identify genes regulated by electroconvulsive shock (ECS) and to observe the pattern of expression of genes according to different developmental stages and brain regions.METHODS:
ECS(130V, 0.5 sec) was given to male Sprague-Dawley rats with age of postnatal day 7 and 21(P7, P21 respectively). After screening genes regulated by ECS with mRNA differential display-PCR(DD-PCR), we selected one clone among them and observed the induction of this gene after ECS by time-dependent Northern blot analysis of rat brain of P7, P21 and adult rat cortex and hippocampus.RESULTS:
By DD-PCR method, we have identified four clones whose expression was regulated by ECS. Among them, one(CP 10-2) was proved to be a new gene by sequencing and BLAST search. Its expression was increased after ECS in P7, P21, and adult rat brain. The expression of CP 10-2 reached peak level at 180 minutes after ECS in P7 rat brain, but was further increased until 360 minutes after ECS in P21 and adult rat brain.CONCLUSION:
In this study, a new gene was identified in rat brain which showed up-regulated expression in response to ECS. Cloning and characterization of this new gene would be helpful to elucidate the effect of ECS in rat brain.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Brain
/
RNA, Messenger
/
Mass Screening
/
Blotting, Northern
/
Clone Cells
/
Rats, Sprague-Dawley
/
Cloning, Organism
/
Electroshock
/
Hippocampus
Type of study:
Screening study
Limits:
Animals
/
Humans
/
Male
Language:
Korean
Journal:
Journal of Korean Neuropsychiatric Association
Year:
2000
Type:
Article
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