Detection of Listeria monocytogenes in CSF from Three Patients with Meningoencephalitis by Next-Generation Sequencing
Journal of Clinical Neurology
;
: 446-451, 2016.
Article
in English
| WPRIM
| ID: wpr-104822
ABSTRACT
BACKGROUND AND PURPOSE:
Encephalitis caused by Listeria monocytogenes (L. monocytogenes) is rare but sometimes fatal. Early diagnosis is difficult using routine cerebrospinal fluid (CSF) tests, while next-generation sequencing (NGS) is increasingly being used for the detection and characterization of pathogens.METHODS:
This study set up and applied unbiased NGS to detect L. monocytogenes in CSF collected from three cases of clinically suspected listeria meningoencephalitis.RESULTS:
Three cases of patients with acute/subacute meningoencephalitis are reported. Magnetic resonance imaging and blood cultures led to a suspected diagnosis of L. monocytogenes, while the CSF cultures were negative. Unbiased NGS of CSF identified and sequenced reads corresponding to L. monocytogenes in all three cases.CONCLUSIONS:
This is the first report highlighting the feasibility of applying NGS of CSF as a diagnostic method for central nervous system (CNS) L. monocytogenes infection. Routine application of this technology in clinical microbiology will significantly improve diagnostic methods for CNS infectious diseases.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Magnetic Resonance Imaging
/
Central Nervous System
/
Cerebrospinal Fluid
/
Communicable Diseases
/
Early Diagnosis
/
Diagnosis
/
Encephalitis
/
Listeria
/
Listeria monocytogenes
/
Meningitis, Listeria
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
English
Journal:
Journal of Clinical Neurology
Year:
2016
Type:
Article
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