Effect of Nitric Oxide on the Survival of R28 Cells
Journal of the Korean Ophthalmological Society
;
: 919-922, 2009.
Article
in Korean
| WPRIM
| ID: wpr-10539
ABSTRACT
PURPOSE:
To evaluate the effect of nitric oxide (NO) on the survival of R28 cells.METHODS:
After immunostaining for GFAP, vimentin, S-100, and neurofilament, R28 cells were exposed to S-nitroso-N-acetyl-D, L-penicillamine (SNAP) at various concentrations, with and without the NO inhibitor, Nomega-Nitro-L-arginine methyl ester (L-NAME) for 1 and 3 days. Cellular survival of R28 cells and the production of NO were quantified by rapid colorimetric assays using the MTT and Griess assay, respectively. To evaluate the effect of serum, 10% serum or serum-free media were used separately.RESULTS:
R28 cells showed strong immunoreactivity to GFAP and vimentin compared to S-100 or neurofilament. SNAP inhibited the survival of R28 cells in a dose-dependent manner, and this effect of NO on the cellular survival was abolished by L-NAME. These results were similar after exposure for 1 and 3 days, regardless of the presence of serum in the media.CONCLUSIONS:
The current results suggest that NO decreased the survival of R28 cells. Further studies are necessary to evaluate the mechanism of cytotoxicity of the R28 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Vimentin
/
Culture Media, Serum-Free
/
NG-Nitroarginine Methyl Ester
/
Nitric Oxide
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2009
Type:
Article
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