Topical application of herbal formula for the treatment of ligature-induced periodontitis
Journal of Periodontal & Implant Science
;
: 145-151, 2015.
Article
in English
| WPRIM
| ID: wpr-10571
ABSTRACT
PURPOSE:
The aim of this study was to investigate the therapeutic effects of a herbal formula, PerioH-035, containing Angelica sinensis, steamed Rehmannia glutinosa, Angelica dahurica, Cimicifuga heracleifolia, and Zanthoxylum piperitum on the periodontal breakdown in a well-established ligature-induced periodontitis model in rats.METHODS:
Sprague-Dawley rats were randomly assigned to 1 of 4 groups NL (non-ligatured), L (ligatured), P1 (ligatured and treated with 1 mg/mL PerioH-035), P100 (ligatured and treated with 100 mg/mL PerioH-035). Periodontitis was induced by placing a ligature around the mandibular first molars. PerioH-035 was topically applied to both sides of the first molar for 2 weeks. The right side of the mandibles was retrieved for micro-computed tomography (CT) and methylene blue staining to analyze alveolar bone loss. The left side of the mandibles was histologically analyzed by TRAP and H&E staining. The MMP-9 mRNA level in gingival tissue was investigated by RT-PCR.RESULTS:
Alveolar bone resorption was significantly reduced in the PerioH-035-treated groups. The number of dense multi-nucleated cells found to be TRAP-positive by staining in the ligatured rats was markedly decreased by PerioH-035 application. In addition, periodontal tissue destruction, especially cementum demineralization, was ameliorated in the P1 and P100 groups. Moreover, gingival tissue from the PerioH-035-treated group showed a decrease in the MMP-9 mRNA level, resulting in recovery of collagen degradation.CONCLUSIONS:
These results suggest that PerioH-035 has therapeutic effects on periodontitis, and thus, PerioH-035 shows promise as a treatment for periodontitis. GRAPHICAL ABSTRACT
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoclasts
/
Periodontitis
/
Plants, Medicinal
/
Steam
/
Bone Resorption
/
RNA, Messenger
/
Alveolar Bone Loss
/
Collagen
/
Rats, Sprague-Dawley
/
Matrix Metalloproteinase 9
Limits:
Animals
Language:
English
Journal:
Journal of Periodontal & Implant Science
Year:
2015
Type:
Article
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