The Effects of High Glucose on Paraquat-induced Cell Injury in Human Retinal Pigment Epithelial Cells
Journal of the Korean Ophthalmological Society
;
: 883-889, 2002.
Article
in Korean
| WPRIM
| ID: wpr-106038
ABSTRACT
PURPOSE:
Functional and structural alterations of retinal pigment epithelial cells were observed in experimental and clinical diabetes. To investigate the effects of high glucose on free radical-induced injury in retinal pigment epithelial cells, we determined the effects of high glucose on activities of antioxidant enzymes and paraquat-induced cytotoxicity in cultured human retinal pigment epithelial (HRPE) cells.METHODS:
Human retinal pigment epithelial (HRPE) cell line (ATCCCRL-2302) was cultured with high glucose (22.4 mM)-and normoglucose (5.6 mM)-contained DMEM for 3 days. The activities of superoxide dismutase (SOD), catalase and GSHPx were assayed by Crapo's method, Aebi's method and GUnzler's method, respectively. Paraquat-induced cytotoxicity was determined by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) method.RESULTS:
CuZn-SOD activity of HRPE cells was decreased by 32% in high glucose (25.4 mM) media compared to normoglucose (5.6 mM) media. But the activities of catalase and GSHPx were not changed by high glucose. The paraquat-induced HRPE cells toxicity was increased by high glucose. Diethydithiocarbamate (DDC), as inhibitor of CuZn-SOD, also potentiated paraquat-induced HRPE cellCONCLUSIONS:
Paraquat-induced HEPE cells injury was potentiated by high glucose and decreased CuZn-SOD activity by high glucose may be some roles in free radical-induced HRPE cell injury.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Paraquat
/
Retinaldehyde
/
Superoxide Dismutase
/
Catalase
/
Cell Line
/
Epithelial Cells
/
Glucose
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2002
Type:
Article
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