Effect of Uteroglobin on cPLA2, COX-2 Expression and ERK Activation in NSCLC Cells / 결핵
Tuberculosis and Respiratory Diseases
;
: 638-645, 2004.
Article
in Korean
| WPRIM
| ID: wpr-106174
ABSTRACT
BACKGROUND:
Uteroglobin is a protein produced by the normal bronchial epithelium and its expression level is lower in non-small cell lung cancer tissues and cell lines. It mainly functions as an anti-inflammatory, and when it is overexpressed in cancer cells, the neoplastic phenotype is antagonized. cPLA2 and COX-2, which are also associated with inflammation, were reported to be related to cancer. The relationship between cPLA2, COX-2 and uteroglobin is unclear. The relationship between uteroglobin and ERK, which is related to cell growth, is also not unclear. This study investigated the changes in the cPLA2 and COX-2 expression levels and the ERK activities after the overexpression of uteroglobin in non-small cell lung cancer cell lines.METHODS:
The A549 and NCI-H460 cell lines were infected by adenovirus-null and adenovirus- uteroglobin. The cChange in the cPLA2, COX-2 expression level and ERK activity after uteroglobin overexpression was measured by Western blot. The change in MMP activity was measured by zymography.RESULTS:
Western blot revealed decreased expression levels of cPLA2, and COX-2, and increased pERK levels in nonsmall cell lung cancer cells after uteroglobin overexpression. Zymography revealed no changes in the MMP-2 activity and lower MMP-9 activity. U0126, which is a specific inhibitor of ERK-activating kinase MEK-1/-2, prevented the decrease in the MMP-9 activityCONCLUSIONS:
A decrease in cPLA2 expression, COX-2 expression, MMP-9 activity and a increase in ERK activity may be related to the anticancer effects of uteroglobin in nonsmall cell lung cancer cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Phosphotransferases
/
Uteroglobin
/
Cell Line
/
Blotting, Western
/
Carcinoma, Non-Small-Cell Lung
/
Epithelium
/
Inflammation
/
Lung Neoplasms
Language:
Korean
Journal:
Tuberculosis and Respiratory Diseases
Year:
2004
Type:
Article
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