Prolonged oral administration of Gastrodia elata extract improves spatial learning and memory of scopolamine-treated rats / 한국실험동물학회지
Laboratory Animal Research
;
: 69-77, 2015.
Article
in English
| WPRIM
| ID: wpr-106543
ABSTRACT
Gastrodia elata (GE) is traditionally used for treatment of various disorders including neurodegenerative diseases such as Alzheimer's disease. To investigate the neuroprotective effect of GE, amyloid-beta peptide (Abeta)-treated PC12 cells were cultured with GE aqueous extract. In vitro assay demonstrated that 50 microM of pre-aggregated Abeta was lethal to about a half portion of PC12 cells and that Abeta aggregate-induced cell death was significantly decreased with GE treatment at < or =10 mg/mL in a dose-dependent manner. To further examine in vivo cognitive-improving effects, an artificial amnesic animal model, scopolamine-injected Sprague-Dawley rats, were orally administered the extract for 6 weeks followed by behavioral tests (the passive avoidance test and Morris water maze test). The results showed that an acute treatment with scopolamine (1 mg/kg of body weight) effectively induced memory impairment in normal rats and that the learning and memory capability of scopolamine-treated rats improved after prolonged administration of GE extract (50, 250 and 500 mg/kg of body weight for 6 weeks). These findings suggest that a GE regimen may potentially ameliorate learning and memory deficits and/or cognitive impairments caused by neuronal cell death.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Scopolamine
/
Body Weight
/
Administration, Oral
/
PC12 Cells
/
Cell Death
/
Rats, Sprague-Dawley
/
Neuroprotective Agents
/
Neurodegenerative Diseases
/
Models, Animal
/
Gastrodia
Limits:
Animals
Language:
English
Journal:
Laboratory Animal Research
Year:
2015
Type:
Article
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