Evaluation of Two Commercial HLA-B27 Real-Time PCR Kits / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 589-593, 2009.
Article
in English
| WPRIM
| ID: wpr-106754
ABSTRACT
BACKGROUND:
The standard PCR with sequence-specific primers (SSP) is a widely used method of HLA-B27 typing in clinical practice. The aim of our study was to evaluate 2 Korean HLA-B27 kits with different real-time PCR chemistries.METHODS:
To validate the accuracy of real-time PCR kits, we selected 28 HLA-B27-positive samples and 33 HLA-B27-negative samples with a wide range of different HLA-B specificities typed by standard PCR-SSP. The 2 real-time PCR kits used were the AccuPower(R) HLA-B27 real-time PCR kit (Bioneer, Korea) with TaqMan probes and the Real-Q(TM) HLA-B*27 detection kit (BioSewoom, Korea) with SYBR Green I dye for melting curve analysis.RESULTS:
All 61 samples typed by PCR-SSP demonstrated a perfect concordance with the 2 real-time PCR assays. It was possible to clearly discriminate between HLA-B27-positive and -negative samples in both real-time assays.CONCLUSIONS:
In summary, both real-time PCR assays for HLA-B27 were fast, reliable, well-adapted for routine laboratory testing, and attractive alternatives to the conventional PCR-SSP method.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organic Chemicals
/
Reagent Kits, Diagnostic
/
Histocompatibility Testing
/
HLA-B27 Antigen
/
Polymerase Chain Reaction
/
Transition Temperature
Limits:
Humans
Language:
English
Journal:
The Korean Journal of Laboratory Medicine
Year:
2009
Type:
Article
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