Improved Antibiotic Susceptibility Test of Orientia tsutsugamushi by Flow Cytometry Using Monoclonal Antibody
Journal of Korean Medical Science
;
: 1-6, 2007.
Article
in English
| WPRIM
| ID: wpr-107141
ABSTRACT
Orientia tsutsugamushi causes scrub typhus, which is endemic in many countries in the Asia-Pacific region including Korea. Recent emergence of doxycycline-resistant strains from Thailand has underlined the importance of the susceptibility tests of O. tsutsugamushi to antibiotics. To improve the flow cytometric technique for the susceptibility test, we applied a monoclonal antibody (MAb) in the quantification of O. tsutsugamushi. With using MAb FS15, we determined the doxycycline susceptibility of two strains, Boryong and AFSC-4 strain which is reported to be doxycycline-sensitive and resistant, respectively. The growth of both strains was inhibited to below 10% of the control in the presence of 0.1 microgram/mL or higher concentrations of doxycycline. We suggest that our approach is more quantitative and reproducible than the conventional microscopic methods.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Orientia tsutsugamushi
/
Microbial Sensitivity Tests
/
Flow Cytometry
/
Antibodies, Monoclonal
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2007
Type:
Article
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