Factor V Leiden mutation in Korean women with pregnancy-induced hypertension / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology
;
: 857-866, 2005.
Article
in Korean
| WPRIM
| ID: wpr-107181
ABSTRACT
OBJECTIVE:
The purposes of this study was to evaluate the frequency of Leiden mutation (missense mutation in the factor V gene at exon 10, 1691 CGA to CAA) in Korean women with well characterized pregnancy-induced hypertension (PIH) compared with normotensive gravid women.METHODS:
Genomic DNA from 121 PIH cases and 98 normotensive pregnant control cases were used for polymerase chain reaction (PCR). To genotype Leiden mutation (missense mutation in the factor V gene, exon 10 (1691 G to A)), primers (5'-TGC CCA GTG CTT AAC AAG ACC A-3', 5'-TGT TAT CAC ACT GGT GCT AA-3') were employed to make 267 base pair (bp) PCR product. There was an initial denaturation at 94 degrees C 5 min, followed by 30 cycles of one minute at 94 degrees C, one minute at 55 degrees C, and one minute at 72 degrees C. A 267 bp PCR product was further digested with Mnl I for 2 hour at 37 degrees C and analysed through 12% polyacrylamide gel electrophoresis to determine genotype. Allele 1691G yielded 37 bp, 67 bp, 163 bp fragment and allele 1691A yielded 67 bp, 200 bp fragment.RESULTS:
We examined the genotypes of factor V of 121 Korean women with pregnant induced hypertension and 98 normal pregnant women. None of the 219 Korean women carried the factor V Leiden mutation.CONCLUSION:
The factor V Leiden mutation is absent and not a common cause of PIH in Korean women.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA
/
Factor V
/
Polymerase Chain Reaction
/
Exons
/
Base Pairing
/
Pregnant Women
/
Hypertension, Pregnancy-Induced
/
Electrophoresis, Polyacrylamide Gel
/
Alleles
/
Genotype
Limits:
Female
/
Humans
/
Pregnancy
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2005
Type:
Article
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