Pregnane X Receptor agonist Increases the Expression Levels of the Plasma Membrane Monoamine Transporter
Translational and Clinical Pharmacology
; : 19-21, 2014.
Article
in En
| WPRIM
| ID: wpr-107310
Responsible library:
WPRO
ABSTRACT
We evaluated the effect of the pregnane X receptor agonist, pregnenolone 16 alpha-carbonitrile (PCN) on the expression levels of plasma monoamine transporter (PMAT) in the intestine. Male C57/BL6 mice were divided into two 2 groups: mice in the PCN group (n=3) were administered PCN once a day for 4 days, while those in the control group (n=3) received the same volume of vehicle once a day for 4 days. After the mice were killed 24 h after administration of the last dose of PCN or vehicle, and the expression levels of PMAT in the intestine tissues were isolated and measured the expression level of PMAT using immunohistochemical and western blotting analyses. The expression level of PMAT expression levels in the small intestine increased after PCN treatment. These results suggest that the induction of PMAT may play a clinically significant role by increasing intestinal absorption of PMAT substrates such as metformin.
Key words
Full text:
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Index:
WPRIM
Main subject:
Plasma
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Pregnenolone Carbonitrile
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Cell Membrane
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Blotting, Western
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Intestinal Absorption
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Intestine, Small
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Intestines
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Metformin
Limits:
Animals
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Humans
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Male
Language:
En
Journal:
Translational and Clinical Pharmacology
Year:
2014
Type:
Article