Differential regulation of inducible nitric oxide synthase and cyclooxygenase-2 expression by superoxide dismutase in lipopolysaccharide stimulated RAW 264.7 cells
Experimental & Molecular Medicine
;
: 629-637, 2009.
Article
in English
| WPRIM
| ID: wpr-10783
ABSTRACT
Inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) have been known to be involved in various pathophysiological processes such as inflammation. This study was performed to determine the regulatory function of superoxide dismutase (SOD) on the LPS-induced expression of iNOS, and COX-2 in RAW 264.7 cells. When a cell-permeable SOD, Tat-SOD, was added to the culture medium of RAW 264.7 cells, it rapidly entered the cells in a dose-dependent manner. Treatment of RAW 264.7 cells with Tat-SOD led to decrease in LPS-induced ROS generation. Pretreatment with Tat-SOD significantly inhibited LPS-induced expression of iNOS and NO production but had no effect on the expression of COX-2 and PGE2 production in RAW 264.7 cells. Tat-SOD inhibited LPS-induced NF-kappaB DNA binding activity, IkappaBalpha degradation and activation of MAP kinases. These data suggest that SOD differentially regulate expression of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Superoxide Dismutase
/
Cell Line
/
Gene Expression Regulation
/
Lipopolysaccharides
/
Cytokines
/
NF-kappa B
/
Reactive Oxygen Species
/
Mitogen-Activated Protein Kinase Kinases
/
Cyclooxygenase 2
/
Nitric Oxide Synthase Type II
Limits:
Animals
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2009
Type:
Article
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