Role of Clusterin and Tumor Necrosis Factor Receptors on the Apoptosis of Prostate Cancer Cells / 대한남성과학회지
Korean Journal of Andrology
;
: 43-52, 2011.
Article
in English
| WPRIM
| ID: wpr-107857
ABSTRACT
PURPOSE:
In prostate cancer, the anti-apoptotic mechanism of sulfated glycoprotein-2 (clusterin) against tumor necrosis factor-alpha (TNF-alpha) receptors and the action of type 2 TNF-alpha receptor (TNFR2) were investigated. MATERIALS ANDMETHODS:
TNF-alpha, agonistic-TNF type 1 receptor (TNFR1) antibody, agonistic-TNF-R2 antibody and their combination were treated in PC3 cell line with or without anti-clusterin. Cytotoxicity was assessed by trypan blue dye exclusion assay. By using flowcytometric analysis, the exact amount of apoptosis and their changes were assessed.RESULTS:
Apoptosis was significantly increased in both agonistic-TNFR1 antibody and TNF-alpha treated cases after blocking the activity of clusterin. The more the anti-clusterin antibody added, the more the apoptosis occurred. The increase of total apoptosis was greater in TNF-alpha treated cells than in agonistic-TNFR1 antibody treated ones. However, there was no increase of apoptosis in agonistic-TNFR2 antibody and TNF-alpha with agonistic-TNFR2 antibody treated cases, respectively.CONCLUSIONS:
Clusterin prevents TNF-alpha induced apoptosis by affecting TNFR1. The difference in degree of apoptosis between agonistic-TNFR1 antibody treated cells and TNF-alpha treated ones suggests the possibility of the action of TNFR2. It may be associated with affinity of TNF-alpha to the tumor cell surface.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prostate
/
Prostatic Neoplasms
/
Trypan Blue
/
Cell Line
/
Tumor Necrosis Factor-alpha
/
Apoptosis
/
Receptors, Tumor Necrosis Factor
/
Receptors, Tumor Necrosis Factor, Type I
/
Receptors, Tumor Necrosis Factor, Type II
/
Diminazene
Language:
English
Journal:
Korean Journal of Andrology
Year:
2011
Type:
Article
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