The Effect of Combined Treatment of Cadmium and Methionine on the Accumulation of Cadmium in Liver and Kidney and the Activation of Alkaline Phosphatase in Blood of Mice / 예방의학회지

ABSTRACT

This research is to examine the detoxication effect of methionine on cadmium intoxication. For this purpose, this paper provides an analysis of the data on the groups of mice (ICR), one group of mice treated with 40 ppm of cadmium only, and other groups of mice combined-treated with cadmium and 0.1%, 0.25%, 0.5% and 1% methionine. After breeding for 40 days, the data on the growth of mice, changes in activation of alkaline phosphatase in blood, and the cadmium content in the liver and kidney, are analysed. The results were as follow 1) The growth-rate of mice, in the cadmium only injected group, was declined by 9% in comparison with the control group after 40 days. But the two groups of cadmium with 0.5% and 1% methionine showed the rise of 9% and 14% respectively above the growth-rate of the control group. The results from the groups of cadmium with 0.1% and 0.25% methionine were similar to that from the cadmium only injected group. 2) Changes in activation of alkaline phosphatase in blood decreased to 86.45% in the cadmium only injected group behind the 100% activation of the control group. The groups of cadmium with 0.1% and 0.25% methionine showed no difference with the former group. But the groups of cadmium with 0.5% and 1% methionine recovered to the 93.14% and 96.08% of activation respectively. 3) The mean content of cadmium in the liver was 0.028+/-0.001 microgram/g in the control group. The cadmium only injected group showed the mean cadmium content of 2.80+/-0.62 microgram/g in the liver, which was similar to 2.82+/-1.03 microgram/g in the group of cadmium with 0.1% methionine, and 2.56+/-0.77 microgram/g in the group of cadmium with 0.25% methionine. But the groups of cadmium with 0.5% and 1% methionine showed the reduction of cadmium contents in the liver to 1.84+/-0.56 microgram/g and 1.74+/-0.35 microgram/g respectively. In the kidney, the groups of cadmium with 0.1%, 0.25% and 0.5% methionine showed the similar cadmium content to the group treated with cadmium only. But the group of cadmium with 1% methionine showed a small increase to 4.13+/-1.00 microgram/g in comparison with the group treated with cadmium only. This analysis proves that the mobility and diffusion of cadmium in the tissues advance faster in the group treated with cadmium and methionine than in the group treated with cadmium only.