Cyclooxygenase 2 in Gastric Carcinoma Is Expressed in Doublecortin- and CaM Kinase-Like-1-Positive Tuft Cells
Gut and Liver
;
: 508-518, 2014.
Article
in English
| WPRIM
| ID: wpr-108130
ABSTRACT
BACKGROUND/AIMS:
Doublecortin and CaM kinase-like-1 (DCAMKL1) is a marker of stem cells expressed predominantly in the crypt base in the intestine. However, DCAMKL1-positive cells have been shown to be differentiated tuft cells rather than quiescent progenitors. Tuft cells are the only epithelial cells that express cyclooxygenase 2 (COX-2) in the normal intestinal epithelium. We previously generated Cdx2-transgenic mice as model mice for intestinal metaplasia and gastric carcinoma. In the current study, we investigated the association between COX-2 and DCAMKL1 in gastric carcinoma.METHODS:
We examined the association between COX-2 and DCAMKL1 expression in gastric carcinomas in clinical samples (early gastric well-differentiated adenocarcinoma) and Cdx2-transgenic mice; and the DCAMKL1-transgenic mouse stomach using immunohistochemistry and quantitative real-time polymerase chain reaction.RESULTS:
The COX-2-expressing cells were scattered, not diffusely expressed, in gastric carcinomas from humans and Cdx2-transgenic mice. DCAMKL1-positive cells were also scattered in the gastric carcinomas, indicating that tuft cells could still be present in gastric carcinoma. COX-2 was expressed in DCAMKL1-positive tuft cells in Cdx2- and DCAMKL1-transgenic mouse stomachs, whereas the Sox9 transcription factor was ubiquitously expressed in gastric carcinomas, including COX-2-positive cells.CONCLUSIONS:
COX-2 is expressed in DCAMKL1-expressing quiescent tuft cells in gastric carcinoma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stomach Neoplasms
/
Mice, Transgenic
/
Adenocarcinoma
/
Protein Serine-Threonine Kinases
/
Intracellular Signaling Peptides and Proteins
/
Epithelial Cells
/
Cyclooxygenase 2
/
SOX9 Transcription Factor
/
Gastric Mucosa
/
Intestinal Mucosa
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Gut and Liver
Year:
2014
Type:
Article
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