Anticolitic Effect of the Rhizome Mixture of Anemarrhena asphodeloides and Coptidis chinensis (AC-mix) in Mice
Biomolecules & Therapeutics
;
: 398-404, 2013.
Article
in English
| WPRIM
| ID: wpr-108272
ABSTRACT
During a screening program to search the anticolitic herbal medicines, 80% ethanol extract of the rhizome of Anemarrhena asphodeloides (AA) was found to potently inhibit the expression of proinflammatory cytokines TNF-alpha and IL-1beta, as well as the activation of NF-kappaB in LPS-stimulated colonic macrophages, followed by that of the rhizome of C. chinensis (CC). AA also potently inhibited TNBS-induced colitic markers, shortening of the colon and increase of macroscopic score, myeloperoxidase activity, TNF-alpha, IL-1beta, and IL-6, in mice. The synergistic effect of CC against the anticolitic effect of AA was investigated. CC synergistically inhibited the anticolitic effect of AA. AC-mix (AA+CC, 11) potently inhibited them. AC-mix also inhibited the activation of NF-kappaB, as well as the expression of TNF-alpha, IL-1beta, IL-6, iNOS and COX-2. The effects of AC-mix against oxazolone-induced colitis were investigated in mice. AC-mix also potently inhibited oxazolone-induced inflammatory markers, colon shortening, macroscopic score, myeloperoxidase activity, NF-kappaB activation and proinflammatory cytokines. Overall, the anti-colitic effect of AC-mix was superior to that of mesalazine. Based on these findings, AC-mix may improve colitis by inhibiting NF-kappaB activation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Mass Screening
/
Cytokines
/
NF-kappa B
/
Interleukin-6
/
Tumor Necrosis Factor-alpha
/
Colitis
/
Colon
/
Peroxidase
/
Mesalamine
/
Rhizome
Type of study:
Prognostic study
/
Screening study
Limits:
Animals
Language:
English
Journal:
Biomolecules & Therapeutics
Year:
2013
Type:
Article
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