The Effect of Quercetin on the BK(Ca) in Umbilical Cord Vein-derived Mesenchymal Stem Cells / 한국실험동물학회지
Laboratory Animal Research
;
: 203-209, 2010.
Article
in Korean
| WPRIM
| ID: wpr-108458
ABSTRACT
Recently, we demonstrated that a large conductance Ca2+-activated K+ channel (BK(Ca)) in human umbilical cord vein-derived mesenchymal cells (hUC-MSCs). In this study, we studied effects of quercetin, the flavonoid used for the modulator of cell cycle and the treatment of anti-tumor, on BK(Ca) in hUC-MSCs using single channel and/or whole cell configuration. Single channel conductance was 277.8+/-36.2 pS in hUC-MSCs and the value is similar to that of typical BK(Ca) channel reported in other cells. The single channel current was activated by increasing of intracellular Ca2+. Quercetin (30~100 micrometer) increased BK(Ca) in whole cell patch configuration whereas EGCG was not affected on the channel. The activation effect of quercetin on BK(Ca) channel was also observed in inside-out configuration. The quercetin-induced BK(Ca) was increased a concentration-dependent manner with an IC50 value of 19.9 micrometer. Taken together, BK(Ca) in hUC-MSCs can be an important target for the action of quercetin and the channels are partly may contribute to maintaining homeostasis by regulation of K ion flux in hUC-MSCs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Quercetin
/
Umbilical Cord
/
Cell Cycle
/
Inhibitory Concentration 50
/
Mesenchymal Stem Cells
/
Homeostasis
Limits:
Humans
Language:
Korean
Journal:
Laboratory Animal Research
Year:
2010
Type:
Article
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