Significance of Epstein-Barr Virus DNA Quantitation in Donors of Hematopoietic Stem Cell Transplantation / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 554-558, 2010.
Article
in Korean
| WPRIM
| ID: wpr-108486
ABSTRACT
BACKGROUND:
Epstein-Barr virus (EBV) is a well-known causative agent of various diseases including post-transplant lymphoproliferative disorders. Although the level of EBV viral load in donors is expected to have a direct effect on recipients after hematopoietic stem cell transplantation (HSCT), little has been studied providing a clear evidence for that. We performed EBV DNA quantitation in donors and analyzed the effect of donors' EBV viral load on the recipients after HSCT.METHODS:
EBV DNA quantitation of peripheral blood in 94 healthy HSCT donors was performed by real-time PCR. We analyzed the distribution of EBV viral load in HSCT donors and EBV positivity in the recipients transplanted from donors who had detectable EBV.RESULTS:
Fifteen HSCT donors (16%) showed positive results in EBV real-time quantitative PCR. EBV viral load was below 500 copies/mL in 5 donors and above 500 (680-11,300) copies/mL in 10 donors. Five of the recipients (33.3%) transplanted from these 15 donors showed positivity in EBV PCR after HSCT. All of the EBV PCR positive recipients were transplanted from donors with viral load of >1,000 copies/mL, and 5 (71%) of 7 donors with viral load of >1,000 copies/mL was associated with posttansplant EBV PCR positivity in the recipients.CONCLUSIONS:
Higher levels of EBV viral load in donors appear to be associated with EBV transmission to recipients in HSCT. EBV real-time quantitative PCR may be needed for screening EBV DNA level in HSCT donors.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tissue Donors
/
Transplantation, Homologous
/
DNA, Viral
/
Polymerase Chain Reaction
/
Herpesvirus 4, Human
/
Hematopoietic Stem Cell Transplantation
/
Viral Load
Limits:
Adolescent
/
Adult
/
Aged
/
Child
/
Female
/
Humans
/
Male
Language:
Korean
Journal:
The Korean Journal of Laboratory Medicine
Year:
2010
Type:
Article
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