Wheatgrass extract inhibits hypoxia-inducible factor-1-mediated epithelial-mesenchymal transition in A549 cells
Nutrition Research and Practice
;
: 83-89, 2017.
Article
in English
| WPRIM
| ID: wpr-108660
ABSTRACT
BACKGROUND/OBJECTIVES:
Epithelial-mesenchymal transition (EMT) is involved in not only cancer development and metastasis but also non-cancerous conditions. Hypoxia is one of the proposed critical factors contributing to formation of chronic rhinosinusitis or nasal polyposis. Wheatgrass (Triticum aestivum) has antioxidant, anti-aging, and anti-inflammatory effects. In this study, we analyzed whether wheatgrass has an inhibitory effect on the EMT process in airway epithelial cells. MATERIALS/METHODS:
A549 human lung adenocarcinoma cells were incubated in hypoxic conditions (CO₂ 5%/O₂ 1%) for 24 h in the presence of different concentrations of wheatgrass extract (50, 75, 100, and 150 µg/mL) and changes in expression of epithelial or mesenchymal markers were evaluated by immunoblotting and immunofluorescence. Accordingly, associated EMT-related transcriptional factors, Snail and Smad, were also evaluated.RESULTS:
Hypoxia increased expression of N-cadherin and reduced expression of E-cadherin. Mechanistically, E-cadherin levels were recovered during hypoxia by silencing hypoxia inducible factor (HIF)-1α or administering wheatgrass extract. Wheatgrass inhibited the hypoxia-mediated EMT by reducing the expression of phosphorylated Smad3 (pSmad3) and Snail. It suppressed the hypoxia-mediated EMT processes of airway epithelial cells via HIF-1α and the pSmad3 signaling pathway.CONCLUSION:
These results suggest that wheatgrass has potential as a therapeutic or supplementary agent for HIF-1-related diseases.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sinusitis
/
Snails
/
Triticum
/
Immunoblotting
/
Adenocarcinoma
/
Cadherins
/
Fluorescent Antibody Technique
/
Epithelial Cells
/
Hypoxia-Inducible Factor 1
/
Epithelial-Mesenchymal Transition
Limits:
Humans
Language:
English
Journal:
Nutrition Research and Practice
Year:
2017
Type:
Article
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