DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B elicits polyfunctional CD8+ T cell responses
Clinical and Experimental Vaccine Research
;
: 235-243, 2014.
Article
in English
| WPRIM
| ID: wpr-108899
ABSTRACT
PURPOSE:
T cell-mediated immune responses, and particularly activation of polyfunctional T cells that simultaneously produce multiple cytokines, are necessary for the control of Mycobacterium tuberculosis. In the present study, we examined if DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B (RpfB) elicits polyfunctional T cell responses in mice. MATERIALS ANDMETHODS:
C57BL/6 mice were immunized intramuscularly three times, at 3-week intervals, with RpfB-expressing plasmid DNA. For comparison, protein immunization was performed with recombinant RpfB in control mice. After immunization, RpfB-specific T cell responses were assessed by interferon-gamma (IFN-gamma) enzyme-linked immunosorbent spot assay and intracellular cytokine staining (ICS), and T cell polyfunctionality was assessed from the ICS data.RESULTS:
RpfB DNA immunization induced not only humoral immune responses, but also CD8+ and CD4+ T cell responses. Immunodominant T-cell epitopes were identified within RpfB by assays with overlapping peptides. RpfB DNA immunization elicited a polyfunctional CD8+ T cell response that was dominated by a functional phenotype of IFN-gamma+/TNF-alpha+/IL-2-/CD107a+.CONCLUSION:
RpfB DNA immunization elicits polyfunctional CD8+ T cell responses, suggesting that RpfB DNA immunization might induce protective immunity against tuberculosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Phenotype
/
Plasmids
/
Tuberculosis
/
DNA
/
T-Lymphocytes
/
Complement Factor B
/
Cytokines
/
Immunization
/
Interferon-gamma
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Clinical and Experimental Vaccine Research
Year:
2014
Type:
Article
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