Alpha-tocopherol Prevents H2O2-induced Tight Junction Occludin Disruption in Blood-Brain Barrier / 체질인류학회지
Korean Journal of Physical Anthropology
;
: 223-233, 2006.
Article
in English
| WPRIM
| ID: wpr-109050
ABSTRACT
Vitamin E is the most important lipid-soluble antioxidant in humans. Although alpha-tocopherol is suggested that it has protective effect from many diseases, little is known about the prevention of occludin alteration in tight junction of blood-brain barrier (BBB) under pathologic insults producing reactive oxygen species (ROSs). In this study, the effects of alpha-tocopherol on H2O2-induced tight junction occludin were studied. Primary culture of rat brain microvessel endothelial cells was investigated with confocal microscopy, Western blot, and cell viability assay. Alpha-tocopherol had no apparent cytotoxicity up to 2.8 mM. The preincubation with alpha-tocopherol suppressed the H2O2-induced cytotoxicity in Alamar Blue assay and phase contrast microscopy. In confocal laser microscopy and Western blot, H2O2-induced loss of occludin was suppressed by preincubation with alpha-tocopherol. The present findings provide evidence that alpha-tocopherol may be beneficial for cellular protection from pathologic insults. Since alpha-tocopherol was demonstrated to have far fewer adverse effects, it would become a noteworthy nutrient or drug for the treatment of neurodegenerative diseases.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Vitamin E
/
Vitamins
/
Brain
/
Blood-Brain Barrier
/
Cell Survival
/
Blotting, Western
/
Microscopy, Phase-Contrast
/
Reactive Oxygen Species
/
Microscopy, Confocal
/
Tight Junctions
Limits:
Animals
/
Humans
Language:
English
Journal:
Korean Journal of Physical Anthropology
Year:
2006
Type:
Article
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