Characterization of sphere-forming HCT116 clones by whole RNA sequencing
Annals of Surgical Treatment and Research
;
: 183-193, 2016.
Article
in English
| WPRIM
| ID: wpr-109194
ABSTRACT
PURPOSE:
To determine CD133+ cells defined as cancer stem cells (CSCs) in colon cancer, we examined whether CD133+ clones in HCT116 demonstrate known features of CSCs like sphere-forming ability, chemodrug-resistance, and metastatic potential.METHODS:
Magnetic cell isolation and cell separation demonstrated that <1% of HCT116 cells expressed CD133, with the remaining cells being CD133- clones. In colon cancer cells, radioresistance is also considered a CSC characteristic. We performed clonogenic assay using 0.4 Gy γ-irradiation.RESULTS:
Interestingly, there were no differences between HCT116 parental and HCT116 CD133+ clones when the cells comprised 0.5% of the total cells, and CD133- clone demonstrated radiosensitive changes compared with parental and CD133+ clones. Comparing gene expression profiles between sphere-forming and nonforming culture conditions of HCT116 subclones by whole RNA sequencing failed to obtain specific genes expressed in CD133+ clones.CONCLUSION:
Despite no differences of gene expression profiles in monolayer attached culture conditions of each clone, sphere-forming conditions of whole HCT116 subclones, parental, CD133+, and CD133- increased 1,761 coding genes and downregulated 1,384 genes related to CSCs self-renewal and survival. Thus, spheroid cultures of HCT116 cells could be useful to expand colorectal CSCs rather than clonal expansion depending on CD133 expressions.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parents
/
Neoplastic Stem Cells
/
RNA
/
Cell Separation
/
Clone Cells
/
Sequence Analysis, RNA
/
Colonic Neoplasms
/
HCT116 Cells
/
Clinical Coding
/
Transcriptome
Limits:
Humans
Language:
English
Journal:
Annals of Surgical Treatment and Research
Year:
2016
Type:
Article
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