Regulation of Inflammatory Repertoires and NF-kappaB Signal Transduction by DDB, an Active Compound from Schizandra Chinensis Baillon
Immune Network
;
: 27-32, 2006.
Article
in Korean
| WPRIM
| ID: wpr-109768
ABSTRACT
BACKGROUND:
Chronic inflammation in the brain has known to be associated with the development of a various neurological diseases including dementia. In general, the characteristic of neuro-inflammation is the activated microglia over the brain where the pathogenesis occurs. Pro-inflammatory repertoires, interleukin-1beta (IL-1beta) and nitric oxide (NO), are the main causes of neuro-degenerative disease, particularly in Alzheimer's disease (AD) which is caused by neuronal destruction. Those pro-inflammatory repertoires may lead the brain to chronic inflammatory status, and thus we hypothesized that chronic inflammation would be inhibited when pro-inflammatory repertoires are to be well controlled by inactivating the signal transduction associated with inflammation.METHODS:
In the present study, we examined whether biphenyl dimethyl dicarboxylate (DDB), an active compound from Schizandra chinensis Baillon, inhibits the NO production by a direct method using Griess reagent and by RT-PCR in the gene expression of inducible nitric oxide synthase ((i)NOS) and IL-1beta. Western blots were also used for the analysis of NF-kappaB and IkappaB.RESULTS:
In the study, we found that DDB effectively inhibited IL-1beta as well as NO production in BV-2 microglial cell, and the translocation of NF-kappaB was comparably inhibited in the presence of DDB comparing those to the positive control, lipopolysaccharide.CONCLUSION:
The data suggested that the DDB from Schizandra chinensis Baillon may play an effective role in inhibiting the pro-inflammatory repertoires which may cause neurodegeneration and the results imply that the compound suppresses a cue signal of the microglial activation which can induce the brain pathogenesis such as Alzheimer's disease.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Brain
/
Signal Transduction
/
Gene Expression
/
Blotting, Western
/
NF-kappa B
/
Microglia
/
Cues
/
Schisandra
/
Dementia
/
Nitric Oxide Synthase Type II
Language:
Korean
Journal:
Immune Network
Year:
2006
Type:
Article
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