Granulocyte Colony Stimulating Factor (G-CSF) Attenuates 2,4,6-Trinitrobenzene Sulfonic Acid (TNBS)-induced Colitis in Mice
Immune Network
;
: 13-19, 2006.
Article
in Korean
| WPRIM
| ID: wpr-109770
ABSTRACT
BACKGROUND:
Granulocyte colony stimulating factor (G-CSF) is known as a cytokine central to the hematopoiesis of blood cells and to modulate their cellular functions. Besides granulocytes and their precursors, monocytes/macrophages and endothelial cells are direct target cells of G-CSF action. G-CSF influences immune cells in an anti-inflammatory way.METHODS:
To evaluate whether G-CSF has a potential for preventing or ameliorating diseases characterized by mucosal inflammation, we used a mouse model with trinitrobenzene sulfonic acid (TNBS)-induced inflammatory colitis. To the mice model G-CSF was administrated daily by intraperitoneal injection. Macroscopic evaluation and immunohistochemical analysis of colonic tissues were performed.RESULTS:
Recombinant human G-CSF significantly inhibited LPS-induced TNF-alpha mRNA expression in THP-1 cells. As for in vivo relevance, G-CSF dramatically reduced the weight loss of mice, colonic damage, and mucosal ulceration that characterize TNBS colitis. Moreover, G-CSF suppressed the expression of tumor necrosis factor-alpha, interleukin-1beta, and intercellular adhesion molecule-1 in TNBS colitis.CONCLUSION:
Current results demonstrate that G-CSF may be an effective agent for the treatment of diseases characterized by mucosal inflammation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ulcer
/
Blood Cells
/
RNA, Messenger
/
Inflammatory Bowel Diseases
/
Weight Loss
/
Granulocyte Colony-Stimulating Factor
/
Colony-Stimulating Factors
/
Tumor Necrosis Factor-alpha
/
Colitis
/
Colon
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Immune Network
Year:
2006
Type:
Article
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