Codelivery of IL-7 Augments Multigenic HCV DNA Vaccine-induced Antibody as well as Broad T Cell Responses in Cynomolgus Monkeys
Immune Network
;
: 198-205, 2010.
Article
in English
| WPRIM
| ID: wpr-10995
ABSTRACT
BACKGROUND:
A crucial limitation of DNA vaccines is its weak immunogenicity, especially in terms of eliciting antibody responses in non-human primates or humans; therefore, it is essential to enhance immune responses to vaccination for the development of successful DNA vaccines for humans.METHODS:
Here, we approached this issue by evaluating interleukin-7 (IL-7) as a genetic adjuvant in cynomolgus monkeys immunized with multigenic HCV DNA vaccine.RESULTS:
Codelivery of human IL-7 (hIL-7)-encoding DNA appeared to increase DNA vaccine-induced antibody responses specific for HCV E2 protein, which plays a critical role in protecting from HCV infection. HCV-specific T cell responses were also significantly enhanced by codelivery of hIL-7 DNA. Interestingly, the augmentation of T cell responses by codelivery of hIL-7 DNA was shown to be due to the enhancement of both the breadth and magnitude of immune responses against dominant and subdominant epitopes.CONCLUSION:
Taken together, these findings suggest that the hIL-7-expressing plasmid serves as a promising vaccine adjuvant capable of eliciting enhanced vaccine-induced antibody and broad T cell responses.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Primates
/
DNA
/
Interleukin-7
/
Vaccination
/
Vaccines, DNA
/
Macaca fascicularis
/
Antibody Formation
Limits:
Humans
Language:
English
Journal:
Immune Network
Year:
2010
Type:
Article
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