Dose-dependent embryotrophic effect of recombinant granulocyte-macrophage colony-stimulating factor and brain-derived neurotrophic factor in culture medium for mouse preimplantation embryo
Obstetrics & Gynecology Science
;
: 373-378, 2014.
Article
in English
| WPRIM
| ID: wpr-110053
ABSTRACT
OBJECTIVE:
To evaluate the dose effect of recombinant mouse granulocyte-macrophage colony-stimulating factor (rmGM-CSF) or brain-derived neurotrophic factor (BDNF) in culture medium on the development of in vitro fertilized mouse embryos.METHODS:
Mature oocytes were retrieved from superovulated female BDF1 mice and inseminated by sperm from male BDF1 mice. On day 1, two-cell stage embryos were divided and cultured until day 5 in the embryo maintenance medium supplemented with 0, 1, 2, 5, or 10 ng/mL of rmGM-CSF or supplemented with 0, 5, 10, or 20 ng/mL of BDNF. Blastocyst formation rate and their cell numbers were assessed.RESULTS:
The blastocyst formation rate and the total cell count in blastocyst was similar in all the rmGM-CSF treatment groups when compared with the control. However, the blastocyst formation rate and the total cell count was significantly higher in the group supplemented with 10 ng/mL of BDNF compared with the control (63.9%, 45.8+/-11.5 vs. 52.3%, 38.0+/-6.8; P<0.05, respectively).CONCLUSION:
Supplementation of 10 ng/mL of BDNF enhanced the developmental potential of mouse preimplantation embryos, but supplementation of rmGM-CSF did not.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oocytes
/
Spermatozoa
/
Blastocyst
/
Cell Count
/
Granulocyte-Macrophage Colony-Stimulating Factor
/
Brain-Derived Neurotrophic Factor
/
Embryonic Structures
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
English
Journal:
Obstetrics & Gynecology Science
Year:
2014
Type:
Article
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