Analysis of p53 Gene Mutation in Basal Cell Carcinoma in Korean Patients / 대한피부과학회지

ABSTRACT

BACKGROUND: There were few data on the gene mutations involved in the development of basal cell carcinoma(BCC) in Korean. OBJECTIVE: To gain insight into the role of p53 gene mutations of BCC in Koreans. METHODS: Fifteen BCCs were screened for mutation of the p53 gene. Immunohistochemical staining was done on the paraffin sections using a labelled streptavidin-biotin-peroxidase complex method with the primary antibody against p53 protein. Analysis of p53 mutation was done by non-isotopic RNase cleavage assay and direct sequencing. RESULTS: Mutation of p53 gene was found in 40%(6/15) of the cases. One case showed mutations in exon 6, one in exon 6 and intron 8, two in exon 8, one in exon 9, and one in intron 5. But ultraviolet specific C->T change was detected in only one case. Immunohistochemical expression of p53 was seen in 40%(6/15), but its expression did not coincide with p53 gene mutation. CONCLUSION: Ultraviolet specific mutations of p53 were much less frequent in Korean than in Caucasian, suggesting other etiologies than ultraviolet radiation.