A novel M2e-multiple antigenic peptide providing heterologous protection in mice
Journal of Veterinary Science
;
: 71-78, 2016.
Article
in English
| WPRIM
| ID: wpr-110763
ABSTRACT
Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Virus Replication
/
Body Weight
/
Influenza Vaccines
/
Vaccines, Synthetic
/
Random Allocation
/
Survival Analysis
/
Orthomyxoviridae Infections
/
Epitopes, T-Lymphocyte
/
Disease Models, Animal
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2016
Type:
Article
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