The Effect of Protein Kinase C Pretreatment on Gliotoxin Induced Apoptosis in H9c2 Cells / 체질인류학회지
Korean Journal of Physical Anthropology
;
: 119-128, 2000.
Article
in Korean
| WPRIM
| ID: wpr-110819
ABSTRACT
Aspergillus funigatus and other pathogenic fungi synthesize a toxic epidithi- odiopiperzine (ETP) metabolite called gliotoxin. Gliotoxin is an epidithiodiopiperzine compound which can both react with sulfhydryl groups and form hydrogen peroxide. The fungal toxin gliotoxin induces apoptotic cell death in a variety of cells. Apoptosis induced by gliotoxin need calcium but effect of calcium preconditioning is unknown by gliotoxin. We studied the effect of protein kinase C and calcium preconditioning on gliotoxin-induced apoptosis in H9c2 cell. PKC and calcium preconditiong inhibited DNA fragmentation by gliotoxin. From this above results suggest that gliotoxin induce apoptosis via caspase-3 activation, because caspase-3 inhibitor (DEVD-CHO) didn't induce apoptosis in gliotoxin treated H9c2 clls. Calcium and PKC preconditioning inhibit caspase-3 activation by gliotoxin. These data means that PKC preconditioning is related with caspase-3 regulate in gliotoxin-induced apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Kinases
/
Aspergillus
/
Protein Kinase C
/
Calcium
/
Cell Death
/
Apoptosis
/
Caspase 3
/
DNA Fragmentation
/
Fungi
/
Gliotoxin
Language:
Korean
Journal:
Korean Journal of Physical Anthropology
Year:
2000
Type:
Article
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