Neuroprotective effects of mild hypoxia in organotypic hippocampal slice cultures / 소아과
Korean Journal of Pediatrics
;
: 142-147, 2015.
Article
in English
| WPRIM
| ID: wpr-111025
ABSTRACT
PURPOSE:
The aim of this study was to investigate the potential effects of mild hypoxia in the mature and immature brain.METHODS:
We prepared organotypic slice cultures of the hippocampus and used hippocampal tissue cultures at 7 and 14 days in vitro (DIV) to represent the immature and mature brain, respectively. Tissue cultures were exposed to 10% oxygen for 60 minutes. Twenty-four hours after this hypoxic insult, propidium iodide fluorescence images were obtained, and the damaged areas in the cornu ammonis 1 (CA1), CA3, and dentate gyrus (DG) were measured using image analysis.RESULTS:
In the 7-DIV group compared to control tissue, hypoxia-exposed tissue showed decreased damage in two regions (CA1 5.59%+/-2.99% vs. 4.80%+/-1.37%, P=0.900; DG 33.88%+/-12.53% vs. 15.98%+/-2.37%, P=0.166), but this decrease was not statistically significant. In the 14-DIV group, hypoxia-exposed tissue showed decreased damage compared to control tissues; this decrease was not significant in the CA3 (24.51%+/-6.05% vs. 18.31%+/-3.28%, P=0.373) or DG (15.72%+/-3.47% vs. 9.91%+/-2.11%, P=0.134), but was significant in the CA1 (50.91%+/-5.90% vs. 32.30%+/-3.34%, P=0.004).CONCLUSION:
Although only CA1 tissues cultured for 14 DIV showed significantly less damage after exposure to hypoxia, the other tissues examined in this study showed a tendency towards less damage after hypoxic exposure. Therefore, mild hypoxia might play a protective role in the brain.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxygen
/
Propidium
/
Brain
/
Neuroprotective Agents
/
Dentate Gyrus
/
Fluorescence
/
Hippocampus
/
Hypoxia
Language:
English
Journal:
Korean Journal of Pediatrics
Year:
2015
Type:
Article
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