Cell response to a newly developed Ti-10Ta-10Nb alloy and its sputtered nanoscale coating
The Journal of Advanced Prosthodontics
;
: 56-61, 2009.
Article
in English
| WPRIM
| ID: wpr-111181
ABSTRACT
STATEMENT OF PROBLEM:
The success of titanium implants is due to osseointegration or the direct contact of the implant surface and bone without a fibrous connective tissue interface.PURPOSE:
The purpose of this study was to evaluate the osteoblast precursor response to titanium - 10 tantalum - 10 niobium (Ti-Ta-Nb) alloy and its sputtered coating. MATERIAL ANDMETHODS:
Ti-Ta-Nb coatings were sputtered onto the Ti-Ta-Nb disks. Ti6-Al-4V alloy disks were used as controls. An osteoblast precursor cell line, were used to evaluate the cell responses to the 3 groups. Cell attachment was measured using coulter counter and the cell morphology during attachment period was observed using fluorescent microscopy. Cell culture was performed at 4, 8, 12 and 16 days.RESULTS:
The sputtered Ti-Ta-Nb coatings consisted of dense nanoscale grains in the range of 30 to 100 nm with alpha-Ti crystal structure. The Ti-Ta-Nb disks and its sputtered nanoscale coatings exhibited greater hydrophilicity and rougher surfaces compared to the Ti-6Al-4V disks. The sputtered nanoscale Ti-Ta-Nb coatings exhibited significantly greater cell attachment compared to Ti-6Al-4V and Ti-Ta-Nb disks. Nanoscale Ti-Ta-Nb coatings exhibited significantly greater ALP specific activity and total protein production compared to the other 2 groups.CONCLUSIONS:
It was concluded that nanoscale Ti-Ta-Nb coatings enhance cell adhesion. In addition, Ti-Ta-Nb alloy and its nanoscale coatings enhanced osteoblast differentiation, but did not support osteoblast precursor proliferation compared to Ti-6Al-4V. These results indicate that the new developed Ti-Ta-Nb alloy and its nanoscale Ti-Ta-Nb coatings may be useful as an implant material.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organothiophosphorus Compounds
/
Osteoblasts
/
Tantalum
/
Titanium
/
Cell Adhesion
/
Cell Line
/
Edible Grain
/
Osseointegration
/
Connective Tissue
/
Cell Culture Techniques
Language:
English
Journal:
The Journal of Advanced Prosthodontics
Year:
2009
Type:
Article
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