Preliminary Study of Tissue Engineered Bladder Regeneration with Poly (epsilon-caprolactone) (PCL) Sheet Seeded with Autologous Muscle-derived Stem Cell / 대한비뇨기과학회지
Korean Journal of Urology
; : 1094-1097, 2005.
Article
in Ko
| WPRIM
| ID: wpr-111367
Responsible library:
WPRO
ABSTRACT
PURPOSE: To investigate the feasibility of using a poly (epsilon-caprolactone) (PCL) sheet seeded with autologous muscle-derived stem cells as a bladder substitute. MATERIALS AND METHODS: Muscle-derived stem cells were isolated from the gastrocnemius muscle of 9 female Sprague-Dawley rats using a preplate technique, and cultured on a 5x5mm PCL sheet. The sheets were implanted into the mesentery of the rats in an autologous manner. Three rats were sacrificed 2, 4 and 8 weeks after implantation, and the morphological changes were assessed by H&E and immunofluorescence staining including DAPI, myosin heavy chain (MHC) and choline acetyl transferase (CAT). RESULTS: All the rats survived for the scheduled time. A mild inflammatory reaction was observed around the PCL sheet in the postoperative 2-week specimen but this receded with time. Muscle cells on the sheet were observed over the experimental period. The 8-week specimen showed a moderate amount of muscle cells on the sheet, and MHC and CAT immunofluorescence staining showed a positive reaction. The muscle layer was not well organized. Angiogenesis was quite noticable between the sheet and the muscle cells on the 8-week specimen. CONCLUSIONS: A PCL sheet seeded with autologous muscle-derived stem cells showed skeletal muscle differentiation on the sheets 8 weeks after mesenteric implantation in an autologous manner. This suggests the feasibility of using a PCL sheet seeded with autologous muscle-derived stem cell as a bladder substitute.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Regeneration
/
Atrophy
/
Stem Cells
/
Transferases
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Urinary Bladder
/
Choline
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Fluorescent Antibody Technique
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Rats, Sprague-Dawley
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Muscle, Skeletal
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Myosin Heavy Chains
Limits:
Animals
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Female
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Humans
Language:
Ko
Journal:
Korean Journal of Urology
Year:
2005
Type:
Article