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Epidemiological Characterizations of Class 1 Integrons from Multidrug-Resistant Acinetobacter Isolates in Daejeon, Korea
Annals of Laboratory Medicine ; : 293-299, 2014.
Article in English | WPRIM | ID: wpr-112276
ABSTRACT

BACKGROUND:

Multidrug-resistant (MDR) Acinetobacter spp. acquire antimicrobial agent-resistance genes via class 1 integrons. In this study, integrons were characterized to investigate the antimicrobial resistance mechanisms of MDR Acinetobacter isolates. In addition, the relationship between the integron type and integron-harboring bacterial species was analyzed by using epidemiological typing methods.

METHODS:

Fifty-six MDR Acinetobacter spp.-A. baumannii (N=30), A. bereziniae (N=4), A. nosocomialis (N=5), and A. pittii (N=17)-were isolated. The minimum inhibitory concentrations (MICs) were determined on the basis of the results of the Epsilometer test (Etest). PCR and DNA sequencing was performed to characterize the gene cassette arrays of class 1 integrons. Multilocus sequence typing (MLST) and repetitive extragenic palindromic sequence (REP)-PCR were performed for epidemiological typing.

RESULTS:

Class 1 integrons were detected in 50 (89.3%) of the 56 isolates, but no class 2 or 3 integron was found within the cohorts. The class 1 integrons were classified into 4 types 2.3-kb type A (aacA4-catB8-aadA1), 3.0-kb type B (aacA4-blaI(MP-1)-bla(OXA-2)), 3.0-kb type C (bla(VIM-2)-aacA7-aadA1), and 1.8-kb type D (aac3-1-bla(OXA-2)-orfD). Type A was most prevalent and was detected only in A. baumannii isolates, except for one A. bereziniae isolate; however, type B was amplified in all Acinetobacter isolates except for A. baumannii isolates, regardless of clone and separation time of the bacteria.

CONCLUSIONS:

Although class 1 integron can be transferred horizontally between unrelated isolates belonging to different species, certain types of class 1 integrons tend to transfer horizontally and vertically among A. baumannii or non-baumannii Acinetobacter isolates.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Acinetobacter / Acinetobacter Infections / DNA, Bacterial / Microbial Sensitivity Tests / Polymerase Chain Reaction / Drug Resistance, Multiple, Bacterial / Acinetobacter baumannii / Integrons / Republic of Korea / Multilocus Sequence Typing Limits: Humans Country/Region as subject: Asia Language: English Journal: Annals of Laboratory Medicine Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Acinetobacter / Acinetobacter Infections / DNA, Bacterial / Microbial Sensitivity Tests / Polymerase Chain Reaction / Drug Resistance, Multiple, Bacterial / Acinetobacter baumannii / Integrons / Republic of Korea / Multilocus Sequence Typing Limits: Humans Country/Region as subject: Asia Language: English Journal: Annals of Laboratory Medicine Year: 2014 Type: Article